Animaniacs
The two Warner Brothers Yakko and Wakko and their Warner sister Dot had been (supposedly) created in the 1930's, but their cartoons were too screwy for the general public to handle. The three Warners were locked up in the studio water tower until they escaped in the 90's. There, they run wild, causing chaos everywhere!

Season 2 · Episode 5 ·
Whistle Stop Mindy
Then, Mindy narrowly escapes danger with the help of her dog, Buttons, in the railroad adventure.
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Episode 5 of season 2 of Animaniacs: “Whistle Stop Mindy”. Aired 1994-09-17. Watch it online free on ZapFlix.